Medaysis Ultra-Sensitive ABL Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the ABL gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type. It is designed to amplify ABL gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.
The feature of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required
| Gene Name | ABL Abelson murine leukemia viral oncogene homolog |
| Catalog | 20 tests: ABL0001-20; 50 tests: ABL0001-50 |
| Official Symbol | ABL |
| Alternative Name | ABL; ABL1; JTK7; Proto-oncogene tyrosine-protein kinase ABL1; c-ABL; p150; c-abl oncogene 1, non-receptor tyrosine kinase |
| GeneID | 25 |
| Uniprot ID | P00519 |
| Target Codon | Exon 4: M244/ Q252 / Y253 / E255 Exon 6: T315/ M351 |
| Other Interested Codons | Exon 4: G250 Exon 6: F317/ F359 |
| Covered Gene Range | Exon 4(A): 237~275 aa Exon 4(B): 184~260 aa Exon 6(A): 308~361 aa Exon 6(B): 310~359 aa |
| List of Components | 1. ABL Exon 4 Primer Set (A) 2. ABL Exon 4 Primer Set (B) 3. ABL Exon 6 Primer Set (A) 4. ABL Exon 6 Primer Set (B) 5. ABL Exon 4 Sequencing Primer-R(A) 6. ABL Exon 4 Sequencing Primer-R(B) 7. ABL Exon 6 Sequencing Primer-R(A) 8. ABL Exon 6 Sequencing Primer-R(B) 9. DNA Quality Control Primer Mix 10. 2x PCR Master Mix 11. Nuclease-Free Water |
| Recommended PCR Condition | 95°C, 10mins; 94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles); 72°C, 10mins°; 10°C, ∞ |
| Platform | PCR + Sanger sequencing assay |
| Shipping Condition | 4°C |
| Long Term Storage | Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles |
| Datasheet | Ultra-Sensitive ABL Mutation Detection Kit |
info@medaysis.com Toll Free: 877-524-9167
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