​​Medaysis Ultra-Sensitive ABL Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the ABL gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type.  It is designed to amplify ABL gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.

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The feature of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required


​Gene NameABL Abelson murine leukemia viral oncogene homolog
Catalog20 tests: ABL0001-20; 50 tests: ABL0001-50
Official SymbolABL
Alternative Name                                                                                             ABL; ABL1; JTK7; Proto-oncogene tyrosine-protein kinase ABL1; c-ABL; p150; c-abl oncogene 1, non-receptor tyrosine kinase
GeneID25
Uniprot IDP00519
Target Codon

Exon 4: M244/ Q252 / Y253 / E255

Exon 6: T315/ M351

Other Interested Codons

Exon 4: G250

Exon 6: F317/ F359

Covered Gene RangeExon 4(A): 237~275 aa
Exon 4(B): 184~260 aa
Exon 6(A): 308~361 aa
Exon 6(B): 310~359 aa
List of Components1. ABL Exon 4 Primer Set (A)
2. ABL Exon 4 Primer Set (B)
3. ABL Exon 6 Primer Set (A)
4. ABL Exon 6 Primer Set (B)
5. ABL Exon 4 Sequencing Primer-R(A)
6. ABL Exon 4 Sequencing Primer-R(B)
7. ABL Exon 6 Sequencing Primer-R(A)
8. ABL Exon 6 Sequencing Primer-R(B)
9. DNA Quality Control Primer Mix
10. 2x PCR Master Mix
11. Nuclease-Free Water
Recommended PCR Condition95°C, 10mins;
94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
72°C, 10mins°;
10°C, ∞
PlatformPCR + Sanger sequencing assay
Shipping Condition4°C
Long Term StorageStable for up to one year when store at -20°C. Avoid freeze/thaw cycles
DatasheetUltra-Sensitive ABL Mutation Detection Kit


                 
 

info@medaysis.com    Toll Free: 877-524-9167

ABL Mutation Detection Kit

Medaysis

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