Medaysis

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AKT3 Mutation Detection Kit

info@medaysis.com    Toll Free: 877-524-9167

​​Medaysis Ultra-Sensitive AKT3 Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the AKT3 gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type.  It is designed to amplify AKT3 gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.


The feature of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required


​Gene Name
AKT3 v-akt murine thymoma viral oncogene homolog 3
Catalog
20 tests: AKT30001-20; 50 tests: AKT30001-50 
Official Symbol
AKT3
Alternative Name                                                                                             
AKT3; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma); RAC-gamma serine/threonine-protein kinase; PKBG; PRKBG; RAC gamma; PKB gamma; RAC-gamma serine/threonine protein kinase; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
GeneID
10000
Uniprot ID
P00519
Target Codon

Exon 2: E17

List of Components1. AKT Exon 2 Primer Set
2. ABL Exon 2 Sequencing Primer-R(A)
3. DNA Quality Control Primer Mix
4. 2x PCR Master Mix
5. Nuclease-Free Water
Recommended PCR Condition95°C, 10mins;
94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
72°C, 10mins°;
10°C, ∞
Platform
PCR + Sanger sequencing assay
Shipping Condition
4°C
Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles
Datasheet
Ultra-Sensitive AKT3 Mutation Detection Kit