Medaysis
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AKT3 Mutation Detection Kit
info@medaysis.com Toll Free: 877-524-9167
Medaysis Ultra-Sensitive AKT3 Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the AKT3 gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type. It is designed to amplify AKT3 gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.
The feature of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required
| Gene Name | AKT3 v-akt murine thymoma viral oncogene homolog 3 |
| Catalog | 20 tests: AKT30001-20; 50 tests: AKT30001-50 |
| Official Symbol | AKT3 |
| Alternative Name | AKT3; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma); RAC-gamma serine/threonine-protein kinase; PKBG; PRKBG; RAC gamma; PKB gamma; RAC-gamma serine/threonine protein kinase; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma |
| GeneID | 10000 |
| Uniprot ID | P00519 |
| Target Codon | Exon 2: E17 |
| List of Components | 1. AKT Exon 2 Primer Set 2. ABL Exon 2 Sequencing Primer-R(A) 3. DNA Quality Control Primer Mix 4. 2x PCR Master Mix 5. Nuclease-Free Water |
| Recommended PCR Condition | 95°C, 10mins; 94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles); 72°C, 10mins°; 10°C, ∞ |
| Platform | PCR + Sanger sequencing assay |
| Shipping Condition | 4°C |
| Long Term Storage | Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles |
| Datasheet | Ultra-Sensitive AKT3 Mutation Detection Kit |