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BRAF Mutation Detection Kit

BRAF, a kinase-encoding gene from the RAS/RAF/MEK/ERK pathway, recently has being studied in all malignant melanomas, including thyroid cancer, malignant melanoma, ovarian cancer, colorectal cancer. The most common mutations in BRAF occur at the valine at residue 600 (V600) within exon 15, and may be a predictive biomarker for limited response to anti-EGFR therapy.​


​Medaysis Ultra-Sensitive BRAF Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the BRAF gene (V600). Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type.  It is designed to amplify BRAF gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.


Features of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required


​Gene Name
BRAF v-raf murine sarcoma viral oncogene homolog B1
Catalog
20 tests: BRAF0001-20; 50 tests: BRAF0001-50
Official Symbol
BRAF
Alternative Name                                                                                             
BRAF; v-raf murine sarcoma viral oncogene homolog B1; serine/threonine-protein kinase B-raf; BRAF1; p94; 94 kDa B-raf protein; proto-oncogene B-Raf; murine sarcoma viral (v-raf) oncogene homolog B1; B-Raf proto-oncogene serine/threonine-protein kinase (p94); NS7; RAFB1; B-RAF1
GeneID
673
Uniprot ID
P15056
Target Codon
Exon 15: V600
Other Interested Codons
Exon 15: D594/ L597/ K601
Covered Gene Range
Exon 15: 591~620 aa
List of Components1. BRAF Exon 15 Primer Set
2. BRAF Exon 15 Sequencing Primer-R
3. DNA Quality Control Primer Mix
4. 2x PCR Master Mix
5. Nuclease-Free Water
Recommended PCR Condition95°C, 10mins;
94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
72°C, 10mins°;
10°C, ∞
Platform
PCR + Sanger sequencing assay
Shipping Condition
4°C
Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles
Datasheet
Ultra-Sensitive BRAF Mutation Detection Kit


 

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