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BRAF Mutation Detection Kit
BRAF, a kinase-encoding gene from the RAS/RAF/MEK/ERK pathway, recently has being studied in all malignant melanomas, including thyroid cancer, malignant melanoma, ovarian cancer, colorectal cancer. The most common mutations in BRAF occur at the valine at residue 600 (V600) within exon 15, and may be a predictive biomarker for limited response to anti-EGFR therapy.
Medaysis Ultra-Sensitive BRAF Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the BRAF gene (V600). Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type. It is designed to amplify BRAF gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.
Features of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required
| Gene Name | BRAF v-raf murine sarcoma viral oncogene homolog B1 |
| Catalog | 20 tests: BRAF0001-20; 50 tests: BRAF0001-50 |
| Official Symbol | BRAF |
| Alternative Name | BRAF; v-raf murine sarcoma viral oncogene homolog B1; serine/threonine-protein kinase B-raf; BRAF1; p94; 94 kDa B-raf protein; proto-oncogene B-Raf; murine sarcoma viral (v-raf) oncogene homolog B1; B-Raf proto-oncogene serine/threonine-protein kinase (p94); NS7; RAFB1; B-RAF1 |
| GeneID | 673 |
| Uniprot ID | P15056 |
| Target Codon | Exon 15: V600 |
| Other Interested Codons | Exon 15: D594/ L597/ K601 |
| Covered Gene Range | Exon 15: 591~620 aa |
| List of Components | 1. BRAF Exon 15 Primer Set 2. BRAF Exon 15 Sequencing Primer-R 3. DNA Quality Control Primer Mix 4. 2x PCR Master Mix 5. Nuclease-Free Water |
| Recommended PCR Condition | 95°C, 10mins; 94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles); 72°C, 10mins°; 10°C, ∞ |
| Platform | PCR + Sanger sequencing assay |
| Shipping Condition | 4°C |
| Long Term Storage | Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles |
| Datasheet | Ultra-Sensitive BRAF Mutation Detection Kit |
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