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​Medaysis Ultra-Sensitive DNMT3A Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the DNMT3A gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type.  It is designed to amplify DNMT3A gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.


Features of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required


​Gene Name
DNMT3A DNA methyltransferase 3A
Catalog
20 tests: DNMT3A0001-20; 50 tests: DNMT3A0001-50
Official Symbol
DNMT3A
Alternative Name                                                                                             
DNMT3A; DNA methyltransferase 3A; DNA (cytosine-5)-methyltransferase 3A; DNA methyltransferase MmuIIIA
GeneID
13435
Uniprot ID
O88508
Target Codon
-
Other Interested Codons
-
Covered Gene Range
-
List of Components1. DNMT3A Primer Set
2. DNMT3A Sequencing Primer-R
3. DNA Quality Control Primer Mix
4. 2x PCR Master Mix
5. Nuclease-Free Water
Recommended PCR Condition95°C, 10mins (x1 cycles)
94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
72°C, 10mins° (x1 cycles)
10°C, ∞
Platform
PCR + Sanger sequencing assay
Shipping Condition
4°C
Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles
Datasheet
Ultra-Sensitive DNMT3A Mutation Detection Kit




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DNMT3A Mutation Detection Kit