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EGFR Mutation Detection Kit

Epidermal growth factor receptor (EGFR) is one of the most frequently amplified, mutated and/or overexpressed genes in human malignancies. Inactivation of EGFR kinase with tyrosine kinase inhibitors (TKIs), such as gefitinib (Iressa) and erlotinib (Tarceva), suppresses signal transduction pathways which control tumor cell growth, proliferation, and resistance to apoptosis. TKIs therapy has shown a significant symptom benefit and prolongs survival on the treatment of Non-Small Cell Lung Cancer (NSCLC) patients with somatic mutations in the EGFR gene. Therefore, detection of the somatic mutations in the EGFR gene may predict a more accurate response of the cancer patients to TKIs therapy.​


Medaysis Ultra-Sensitive EGFR Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the EGFR gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type.  It is designed to amplify CFDNA EGFR gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, fine needle biopsies (FNA), pleural effusion specimens, or and plasma samples. 


The feature of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required


​Gene Name
EGFR epidermal growth factor receptor
Catalog
20 tests: EGFR0001-20; 50 tests: EGFR0001-50
Official Symbol
EGFR
Alternative Name                                                                                             
EGFR; Epidermal growth factor receptor; erbb 1; Erbb; Erbb1; ERBB1; HER1; mENA; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; Receptor tyrosine protein kinase ErbB 1; Receptor tyrosine protein kinase ErbB1; Receptor tyrosineprotein kinase ErbB-1; Urogastrone; wa2; Wa5; proto-oncogene c-ErbB -1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; ERBB; HER1; PIG61
GeneID
1956
Uniprot ID
P00533
Target Codon
Exon 18: G719
Exon 19: Exon 19 insertion/ deletion
Exon 20: S768 / Insertion mutation/ T790
Exon 21: L858 / L861
Other Interested Codons
Exon 18: L692/ P699/ Y693/ E709/ any insertion and deletion between 688~728 aa
Exon 19: any insertion and deletion between 739~760 aa
Exon 20: V769/ D770/ P772/ H773/ V774/ R776/ C797/ any insertion and deletion between 761~823 aa
Exon 21: any insertion and deletion between 850~875 aa
Covered Gene Range
Exon 18: 688~728 aa;
Exon 19: 739~760 aa;
Exon 20: 761~823 aa;
Exon 20 T790: 782~823 aa;Exon 21: 850~875 aa
List of Components1. EGFR Exon 18 PCR Primer Mix
2. EGFR Exon 19 PCR Primer Mix
3. EGFR Exon 20 PCR Primer Mix
4. EGFR Exon 21 PCR Primer Mix
5. EGFR Exon 20 T790 PCR Primer Mix
6. EGFR Exon 18 Sequencing Primer-r
7. EGFR Exon19 Sequencing Primer-r
8. EGFR Exon 20 Sequencing Primer-r
9. EGFR Exon 21 Sequencing Primer-r
10. EGFR Exon 20 T790 Sequencing Primer-r
11. DNA Quality Control Primer Mix
12. 2x PCR Master Mix
13. EGFR Exon 19 wild type plasmid
14. EGFR Exon 20 wild-type plasmid 
15. EGFR Exon 21 wild-type plasmid
16. EGFR Exon 19 deletion plasmid 
17. Nuclease-Free Water
Recommended PCR Condition95°C, 10mins (x1 cycles)
94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
72°C, 10mins° (x1 cycles)
10°C, ∞
Platform
PCR + Sanger sequencing assay
Shipping Condition
4°C
Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles
Datasheet
Ultra-Sensitive EGFR Mutation Detection Kit


 

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