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Medaysis Ultra-Sensitive FLT3 Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the FLT3 gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type. It is designed to amplify FLT3 gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.
The feature of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required
Gene Name | FLT3 fms-related tyrosine kinase 3 |
Catalog | 20 tests: FLT30001-20; 50 tests: FLT30001-50 |
Official Symbol | FLT3 |
Alternative Name | FLT3; fms-related tyrosine kinase 3; receptor-type tyrosine-protein kinase FLT3; CD135; FLK2; STK1; STK-1; CD135 antigen; FL cytokine receptor; fetal liver kinase 2; fms-like tyrosine kinase 3; stem cell tyrosine kinase 1; growth factor receptor tyrosine |
GeneID | 2322 |
Uniprot ID | P36888 |
Target Codon | Exon 20: D835 |
Other Interested Codons | Exon 20: I836 |
Covered Gene Range | Exon 20: 807~844 aa |
List of Components | 1. FLT3 Exon 20 Primer Set 2. FLT3 Exon 20 Sequencing Primer-R 3. DNA Quality Control Primer Mix 4. 2x PCR Master Mix 5. Nuclease-Free Water |
Recommended PCR Condition | 95°C, 10mins; 94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles) 72°C, 10mins°; 10°C, ∞ |
Platform | PCR + Sanger sequencing assay |
Shipping Condition | 4°C |
Long Term Storage | Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles |
Datasheet | Ultra-Sensitive FLT3 Mutation Detection Kit |