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FLT3 Mutation Detection Kit

Medaysis

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​Medaysis Ultra-Sensitive FLT3 Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the FLT3 gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type.  It is designed to amplify FLT3 gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.


The feature of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required


​Gene Name
FLT3 fms-related tyrosine kinase 3
Catalog
20 tests: FLT30001-20; 50 tests: FLT30001-50
Official Symbol
FLT3
Alternative Name                                                                                             
FLT3; fms-related tyrosine kinase 3; receptor-type tyrosine-protein kinase FLT3; CD135; FLK2; STK1; STK-1; CD135 antigen; FL cytokine receptor; fetal liver kinase 2; fms-like tyrosine kinase 3; stem cell tyrosine kinase 1; growth factor receptor tyrosine
GeneID
2322
Uniprot ID
P36888
Target Codon
Exon 20: D835
Other Interested Codons
Exon 20: I836
Covered Gene Range
Exon 20: 807~844 aa
List of Components1. FLT3 Exon 20 Primer Set
2. FLT3 Exon 20 Sequencing Primer-R
3. DNA Quality Control Primer Mix
4. 2x PCR Master Mix
5. Nuclease-Free Water

Recommended PCR Condition95°C, 10mins;
94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles)
72°C, 10mins°;
10°C, ∞
Platform
PCR + Sanger sequencing assay
Shipping Condition
4°C
Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles
Datasheet
Ultra-Sensitive FLT3 Mutation Detection Kit


                 
 

1. FLT3 Exon 20 Primer Set
2. FLT3 Exon 20 Sequencing Primer-R
3. DNA Quality Control Primer Mix
4. 2x PCR Master Mix
5. Nuclease-Free Water