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FLT3 Mutation Detection Kit

Medaysis

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Medaysis Ultra-Sensitive FLT3 Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the FLT3 gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type. It is designed to amplify FLT3 gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.

The feature of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required

Gene Name	FLT3 fms-related tyrosine kinase 3
Catalog	20 tests: FLT30001-20; 50 tests: FLT30001-50
Official Symbol	FLT3
Alternative Name	FLT3; fms-related tyrosine kinase 3; receptor-type tyrosine-protein kinase FLT3; CD135; FLK2; STK1; STK-1; CD135 antigen; FL cytokine receptor; fetal liver kinase 2; fms-like tyrosine kinase 3; stem cell tyrosine kinase 1; growth factor receptor tyrosine
GeneID	2322
Uniprot ID	P36888
Target Codon	Exon 20: D835
Other Interested Codons	Exon 20: I836
Covered Gene Range	Exon 20: 807~844 aa
List of Components	1. FLT3 Exon 20 Primer Set 2. FLT3 Exon 20 Sequencing Primer-R 3. DNA Quality Control Primer Mix 4. 2x PCR Master Mix 5. Nuclease-Free Water
Recommended PCR Condition	95°C, 10mins; 94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles) 72°C, 10mins°; 10°C, ∞
Platform	PCR + Sanger sequencing assay
Shipping Condition	4°C
Long Term Storage	Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles
Datasheet	Ultra-Sensitive FLT3 Mutation Detection Kit

1. FLT3 Exon 20 Primer Set
2. FLT3 Exon 20 Sequencing Primer-R
3. DNA Quality Control Primer Mix
4. 2x PCR Master Mix
5. Nuclease-Free Water