Medaysis
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Medaysis Ultra-Sensitive HRAS Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the HRAS gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type. It is designed to amplify HRAS gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.
The feature of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required
| Gene Name | HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog |
| Catalog | 20 tests: HRAS0001-20; 50 tests: HRAS001-50 |
| Official Symbol | HRAS |
| Alternative Name | HRAS; v-Ha-ras Harvey rat sarcoma viral oncogene homolog; HRAS1; GTPase HRas; p21ras; H-Ras- 1; p19 H-RasIDX protein; c-has/bas p21 protein; transforming protein p21; Ha-Ras1 proto-oncoprotein; c-ras-Ki-2 activated oncogene; GTP- and GDP-binding peptide B; transformation gene: oncogene HAMSV; Ras family small GTP binding protein H-Ras; CTLO; HAMSV; K-RAS; N-RAS; RASH1; C-HRAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1 |
| GeneID | 3265 |
| Uniprot ID | P01112 |
| Target Codon | Exon 2: G12 / G13 Exon 3: A59 / Q61 Exon 4: K117 / A146 |
| Other Interested Codons | - |
| Covered Gene Range | Exon 2: 6~37 aa Exon 3: 53~97 aa Exon 4(A): 112~150 aa Exon 4(B): 127~150 aa |
| List of Components | 1. HRAS Exon 2 Primer Set 2. HRAS Exon 3 Primer Set 3. HRAS Exon 4 Primer Set (A) 4. HRAS Exon 4 Primer Set (B) 5. HRAS Exon 2 Sequencing Primer-R 6. HRAS Exon 3 Sequencing Primer-R 7. HRAS Exon 4 Sequencing Primer-R (A) 8. HRAS Exon 4 Sequencing Primer-R (B) 9. DNA Quality Control Primer Mix 10. 2x PCR Master Mix 11. Nuclease-Free Water |
| Recommended PCR Condition | 95°C, 10mins; 94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles); 72°C, 10mins°; 10°C, ∞ |
| Platform | PCR + Sanger sequencing assay |
| Shipping Condition | 4°C |
| Long Term Storage | Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles |
| Datasheet | Ultra-Sensitive HRAS Mutation Detection Kit |
HRAS Mutation Detection Kit
info@medaysis.com Toll Free: 877-524-9167