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​Medaysis Ultra-Sensitive HRAS Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the HRAS gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type.  It is designed to amplify HRAS gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.


The feature of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required


​Gene Name
HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Catalog
20 tests: HRAS0001-20; 50 tests: HRAS001-50
Official Symbol
HRAS
Alternative Name                                                                                             
HRAS; v-Ha-ras Harvey rat sarcoma viral oncogene homolog; HRAS1; GTPase HRas; p21ras; H-Ras- 1; p19 H-RasIDX protein; c-has/bas p21 protein; transforming protein p21; Ha-Ras1 proto-oncoprotein; c-ras-Ki-2 activated oncogene; GTP- and GDP-binding peptide B; transformation gene: oncogene HAMSV; Ras family small GTP binding protein H-Ras; CTLO; HAMSV; K-RAS; N-RAS; RASH1; C-HRAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1
GeneID
3265
Uniprot ID
P01112
Target Codon
Exon 2: G12 / G13
Exon 3: A59 / Q61
Exon 4: K117 / A146
Other Interested Codons
-
Covered Gene Range
Exon 2: 6~37 aa
Exon 3: 53~97 aa
Exon 4(A): 112~150 aa
Exon 4(B): 127~150 aa
List of Components1. HRAS Exon 2 Primer Set
2. HRAS Exon 3 Primer Set
3. HRAS Exon 4 Primer Set (A)
4. HRAS Exon 4 Primer Set (B)
5. HRAS Exon 2 Sequencing Primer-R
6. HRAS Exon 3 Sequencing Primer-R
7. HRAS Exon 4 Sequencing Primer-R (A)
8. HRAS Exon 4 Sequencing Primer-R (B)
9. 2x PCR Master Mix
10. Nuclease-Free Water
Recommended PCR Condition95°C, 10mins;
94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
72°C, 10mins°;
10°C, ∞
Platform
PCR + Sanger sequencing assay
Shipping Condition
4°C
Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles
Datasheet
Ultra-Sensitive HRAS Mutation Detection Kit

HRAS Mutation Detection Kit

info@medaysis.com    Toll Free: 877-524-9167