Medaysis Ultra-Sensitive IDH1 Mutation Detection Kit is a PCR-based test to enrich somatic mutations of IDH1 gene in DNA.
With direct sanger sequencing, This IDH1 Mutation Detection Kit is able to detect 1% somatic mutation from DNA derived from formalin-fixed paraffin-embedded (FFPE) tissues, fine needle biopsy or pleural effusion specimens.
The feature of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required
| Gene Name | IDH1 Isocitrate dehydrogenase 1 |
| Catalog | 20 tests: IDH10001-20; 50 tests: IDH10001-50 |
| Official Symbol | IDH1 |
| Alternative Name | Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase |
| GeneID | 3417 |
| Uniprot ID | O75874 |
| Target Codon | R132 |
| Covered Gene Range | |
| Lists of Components | 1. IDH1 Exon 4 Primer Set 2. IDH2 Exon 4 Sequencing Primer-R 3. DNA Quality Control Primer Mix 4. 2x PCR Master Mix 5. Nuclease-Free Water |
| Recommended PCR Condition | 95°C, 10mins; 94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles); 72°C, 10mins; 10°C, ∞ |
| Platform | PCR + Sanger sequencing assay |
| Shipping Condition | 4°C |
| Long Term Storage | Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles |
| Datasheet | Ultra-Sensitive IDH1 Mutation Detection Kit |
IDH1 Mutation Detection Kit
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