MedaFluo™ Mouse on Mouse Immunofluorescence Detection                                                                                         SDS

MedaFluo™ Mouse on Human, Rabbit, Goat Immunofluorescence Detection                                                               SDS

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MedaFluo™ Rabbit on Human, Rabbit, Goat Immunofluorescence Detection                                                                SDS

​MedaFluo™ Goat on Human Immunofluorescence Detection                                                                                           SDS

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 MedaFluo™ Mouse on Rat Immunofluorescence Detection                                                                                              SDS

MedaFluo™ Rat on Mouse Immunofluorescence Detection                                                                                                SDS                                

MedaFluo™ Non-biotin Immunofluorescence Detection Kits
Mouse, Rabbit, Rat Antibodies on Human and Animal Tissues or Cell Smears

MedaFluo™ immunofluorescence detection kits are specially designed for immunofluorescence detection on formalin-fixed paraffin embedded tissues, cryostat sections, and cell smears. The detection components have gone through extensive absorption to reduce cross reactivity. The kits are utilizing biotin-free signal amplification technologies that eliminate endogenous biotin background to achieve optimal signal-to- noise ratio. MedaFluo™ utilize different conjugating technology thus may be used for multiplexing. Medaysis provides bright and stable Fluor dyes in all detection systems. Fluor dyes exhibit bright fluorescence intensity and photo stability. Also it remains highly fluorescent over a broad pH range (pH 4-9). These make it an ideal choice for researchers' immunofluorescence applications. 

Features

• Non-biotin signal amplification
  
• Cleaner staining without background
• Brighter fluorescence signal
• Improved photo- stability
• Easy to follow protocol
  

MedaFluo™ Mounting Medium
MedaFluo™ Mounting Medium (catalog number MM001M (15ml) and MM001L (100ml)) is an aqueous based mounting medium for preserving fluorescence staining signal on tissue section and cell smears. It contains anti-fade agent and prevents rapid photobleaching of fluorophores. It is compatible with Medaysis dyes,  DyLight®, FITC, Texas Red, AMCA, Cy®, Alexa Fluor®, fluorescent nuclear stains, fluorescent proteins, fluorescent tracers, and most other fluorophores. The fluorescence is retained during prolonged storage at 4ºC in the dark. It does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, R-PE, PC and APC. 
MedaFluo™ Mounting Medium with DAPI (catalog number MM002M (15ml) and MM002L (100ml)) is fortified with DAPI (4,6-diamidino-2-phenylindole) which counterstains DNA or RNA. This product is to be used in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence.

Features

• Antifade agents prevent photobleaching
  
• Prolonged storage without loss of fluorescent signals
• Optically clear with a refractive index of ~1.40
  

info@medaysis.com    Toll Free: 877-524-9167

MedaFluo™ Autofluorescence Blocking Kit

Autofluorescence, caused from endogenous fluorphore such as porphyrins, lipofuscin, NADPH, flavin, collagen, elastin, tryptophan, tyrosine and phenylalanine etc., is an intrinsic property of cells and tissues. It can also be caused by the fixatives used. Autofluorescence complicates the use of fluorescence microscopy and interferes with detection of specific fluorescent signals.
Medaysis ready-to-use  MedaFluo™ Autotofluorescence Blocking Kit (catalog number MB001L (100ml) and MB001M (18ml))will reduce  or eliminate autofluorescence and increase the signal to noise ratio. With the Autofluorescence Blocking Reagent,   researchers   can   clearly    visualize and quantify the target signal without interference from the autofluorescence.

Features

• Clean staining no autofluorescence
  
• Increased signal to noise ratio
• User friendly ready- to-use format
  
• Compatible with commercial fluor
  

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A                                                         B                                                         C                                                         D

A. Mouse anti-smooth muscle actin stain on FFPE mouse tissue. MedaFluo™ MF001
B. Mouse anti-estrogen receptor Stain on FFPE human breast cancer tissue. MedaFluo™ MF031
C. Rabbit Anti-Insulin stain on FFPE human pancreas tissue. MedaFluo™ MF041
D. Mouse anti-actin smooth muscle stain on FFPE rat kidney tissue. MedaFluo™ MF012

A. Stained FFPE pancreas without Autofluorescence Blocking Reagent.

B. Stain FFPE  pancreas tissue with Autofluorescence Blocking Reagent.  

No primary antibodies and no detection systems were added.  Rinse slides with distilled water and mount with fluorescence mounting medium with DAPI.