MedaFluo™ Mouse on Mouse Immunofluorescence Detection                                                                                         SDS


Cat.No
Description
Size
Antibody
Applicable Tissue
Datasheet

MF001M

MF001S

MedaFluo™ 488 Mouse on Mouse Immunofluorescence Detection Kit

18ml

6ml

Mouse
Mouse
MF001
MF002M
MF002S
MedaFluo™ 550 Mouse on Mouse Immunofluorescence Detection Kit
18ml
6ml
Mouse
Mouse
MF002

MF003M

MF003S

MedaFluo™ 594 Mouse on Mouse Immunofluorescence Detection Kit

18ml

6ml

Mouse
Mouse
MF003

MF004M

MF004S

MedaFluo™ 650 Mouse on Mouse Immunofluorescence Detection Kit

18ml

6ml

Mouse
Mouse
MF004








    


MedaFluo™ Mouse on Human, Rabbit, Goat Immunofluorescence Detection                                                               SDS


Cat.No.
Description
Size
Antibody
Applicable Tissue
Datasheet

MF031M

MF031S

MedaFluo™ 488 Immunofluorescence Mouse Detection Kit

18ml

6ml

Mouse
Human, Rabbit, Goat
MF031

MF032M

MF032S

MedaFluo™ 550 Immunofluorescence Mouse Detection Kit

18ml

6ml

Mouse
Human, Rabbit, Goat
MF032

MF033M

MF033S

MedaFluo™ 594 Immunofluorescence Mouse Detection Kit

18ml

6ml

Mouse
Human, Rabbit, Goat
MF033

MF034M

MF034S

MedaFluo™ 650 Immunofluorescence Mouse Detection Kit

18ml

6ml

Mouse
Human, Rabbit, Goat
MF034



                                                           

MedaFluo™ Rabbit on Human, Rabbit, Goat Immunofluorescence Detection                                                                SDS


Cat.No.
Description
Size
Antibody
Applicable Tissue
Datasheet

MF041M

MF041S

MedaFluo™ 488 Immunofluorescence Rabbit Detection Kit

18ml

6ml

Rabbit
Human , Rabbit, Goat
MF041

MF042M

MF042S

MedaFluo™ 550 Immunofluorescence Rabbit Detection Kit

18ml

6ml

Rabbit
Human , Rabbit, Goat
MF042

MF043M

MF043S

MedaFluo™ 594 Immunofluorescence Rabbit Detection Kit

18ml

6ml

Rabbit
Human , Rabbit, Goat
MF043

MF044M

MF044S

MedaFluo™ 650 Immunofluorescence Rabbit Detection Kit

18ml

6ml

Rabbit
Human , Rabbit, Goat
MF044



   




MedaFluo™ Goat on Human Immunofluorescence Detection                                                                                           SDS


Cat.No.
Description
Size
Antibody
Applicable Tissue
Datasheet
MF051M
MF051S
MedaFluo™ 488 Immunofluorescence Goat Detection Kit

18ml

6ml

Goat
Human
MF051

MF052M

MF052S

MedaFluo™ 550 Immunofluorescence Goat Detection Kit

18ml

6ml

Goat
Human
MF052

MF053M

MF053S

MedaFluo™ 594 Immunofluorescence Goat Detection Kit

18ml

6ml

Goat
Human
MF053

MF054M

MF054S

MedaFluo™ 650 Immunofluorescence Goat Detection Kit

18ml

6ml

Goat
Human
MF054


    

  


 MedaFluo™ Mouse on Rat Immunofluorescence Detection                                                                                               SDS


Cat.No.
Description
Size
Antibody
Applicable Tissue
Datasheet

MF011M

MF011S

MedaFluo™ 488 Mouse on Rat Immunofluorescence Detection Kit

18ml

6ml

Mouse
Rat
MF011

MF012M

MF012S

MedaFluo™ 550 Mouse on Rat Immunofluorescence Detection Kit

18ml

6ml

Mouse
Rat
MF012

MF013M

MF013S

MedaFluo™ 594 Mouse on Rat Immunofluorescence Detection Kit

18ml

6ml

Mouse
Rat
MF013

MF014M

MF014S

MedaFluo™ 650 Mouse on Rat Immunofluorescence Detection Kit

18ml

6ml

Mouse
Rat
MF014




      

MedaFluo™ Rat on Mouse Immunofluorescence Detection                                                                                                SDS                                

Cat.No.
Description
Size
Antibody
Applicable Tissue
Datasheet

MF021M

MF021S

MedaFluo™ 488 Rat on Mouse Immunofluorescence Detection Kit

18ml

6ml

Rat
Mouse
MF021

MF022M

MF022S

MedaFluo™ 550 Rat on Mouse Immunofluorescence Detection Kit

18ml

6ml

Rat
Mouse
MF022

MF023M

MF023S

MedaFluo™ 594 Rat on Mouse Immunofluorescence Detection Kit

18ml

6ml

Rat
Mouse
MF023

MF024M

MF024S

MedaFluo™ 650 Rat on Mouse Immunofluorescence Detection Kit

18ml

6ml

Rat
Mouse
MF024

MedaFluo™ Non-biotin Immunofluorescence Detection Kits
Mouse, Rabbit, Rat Antibodies on Human and Animal Tissues or Cell Smears

MedaFluo™ immunofluorescence detection kits are specially designed for immunofluorescence detection on formalin-fixed paraffin embedded tissues, cryostat sections, and cell smears. The detection components have gone through extensive absorption to reduce cross reactivity. The kits are utilizing biotin-free signal amplification technologies that eliminate endogenous biotin background to achieve optimal signal-to- noise ratio. MedaFluo™ utilize different conjugating technology thus may be used for multiplexing. Medaysis provides bright and stable Fluor dyes in all detection systems. Fluor dyes exhibit bright fluorescence intensity and photo stability. Also it remains highly fluorescent over a broad pH range (pH 4-9). These make it an ideal choice for researchers' immunofluorescence applications. 


Dyes Specifications

*Excitation and emission maxima in nanometers (+/- 4nm) in  (PBS)

Emission
Fluor
Excitation/Emission*
Spectrally Similar Dyes
Green
488
493/518
Alexa Fluor 488, FITC, DyLight 488
Yellow
550
562/576
Alexa 546, 555, Cy3,TRITC, DyLight 550
Red
594
593/618
Alexa Fluor 594, Texas Red, DyLight 594
Deep Red
650
652/672
Alexa Fluor 647, Cy5, DyLight 649

Features


  • Non-biotin signal amplification
  • Cleaner staining without background
  • Brighter fluorescence signal
  • Improved photo- stability
  • Easy to follow protocol

MedaFluo™ Mounting Medium
MedaFluo™ Mounting Medium (catalog number MM001M (15ml) and MM001L (100ml)) is an aqueous based mounting medium for preserving fluorescence staining signal on tissue section and cell smears. It contains anti-fade agent and prevents rapid photobleaching of fluorophores. It is compatible with Medaysis dyes,  DyLight®, FITC, Texas Red, AMCA, Cy®, Alexa Fluor®, fluorescent nuclear stains, fluorescent proteins, fluorescent tracers, and most other fluorophores. The fluorescence is retained during prolonged storage at 4ºC in the dark. It does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, R-PE, PC and APC. 
MedaFluo™ Mounting Medium with DAPI (catalog number MM002M (15ml) and MM002L (100ml)) is fortified with DAPI (4,6-diamidino-2-phenylindole) which counterstains DNA or RNA. This product is to be used in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence.


Features

  • Antifade agents prevent photobleaching
  • Prolonged storage without loss of fluorescent signals
  • Optically clear with a refractive index of ~1.40

info@medaysis.com    Toll Free: 877-524-9167

MedaFluo™ Autofluorescence Blocking Kit

Autofluorescence, caused from endogenous fluorphore such as porphyrins, lipofuscin, NADPH, flavin, collagen, elastin, tryptophan, tyrosine and phenylalanine etc., is an intrinsic property of cells and tissues. It can also be caused by the fixatives used. Autofluorescence complicates the use of fluorescence microscopy and interferes with detection of specific fluorescent signals.
Medaysis ready-to-use  MedaFluo™ Autotofluorescence Blocking Kit (catalog number MB001L (100ml) and MB001M (18ml))will reduce  or eliminate autofluorescence and increase the signal to noise ratio. With the Autofluorescence Blocking Reagent,   researchers   can   clearly    visualize and quantify the target signal without interference from the autofluorescence.


Features

  • Clean staining no autofluorescence
  • Increased signal to noise ratio
  • User friendly ready- to-use format
  • Compatible with commercial fluor

Medaysis

Enable Innovation

A                                                         B                                                         C                                                         D

A. Mouse anti-smooth muscle actin stain on FFPE mouse tissue. MedaFluo™ MF001
B. Mouse anti-estrogen receptor Stain on FFPE human breast cancer tissue. MedaFluo™ MF031
C. Rabbit Anti-Insulin stain on FFPE human pancreas tissue. MedaFluo™ MF041
D. Mouse anti-actin smooth muscle stain on FFPE rat kidney tissue. MedaFluo™ MF012

A. Stained FFPE pancreas without Autofluorescence Blocking Reagent.

B. Stain FFPE  pancreas tissue with Autofluorescence Blocking Reagent.  


No primary antibodies and no detection systems were added.  Rinse slides with distilled water and mount with fluorescence mounting medium with DAPI.