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A. Stained FFPE pancreas without Autofluorescence Blocking Reagent.

B. Stain FFPE  pancreas tissue with Autofluorescence Blocking Reagent.  

No primary antibodies and no detection systems were added.  Rinse slides with distilled water and mount with fluorescence mounting medium with DAPI.

Features

• Simultaneous detection of two antigens
  
• Non-biotin signal amplification
  
• Cleaner staining without background
  
• Brighter & stable fluorescence signal
  
• Improved photo-stability
  
• Easy to follow protocol with RTU reagents in dropper bottles
  

DuoFluo™ Non-biotin IF DS Detection Kits                                                                                                                            SDS

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Human color cancer FFPE stained by mouse anti-p53 & rabbit anti-CEA, detected by  DF0001 (DuoFuo™ 488+550 Mouse+Rabbit on Human) simultaneously. Left: Green shows clear luminal membrane and cytoplastic staining of rabbit anti-CEA. Middle: Red shows clear nuclear staining of mouse anti-P53. Right: Color combination. Green: rabbit anti-CEA; Red: mouse anti-P53; Blue: DAPI nuclear counterstain.

MedaFluo™ Mounting Medium
MedaFluo™ Mounting Medium (catalog number MM001M (15ml) and MM001L (100ml)) is an aqueous based mounting medium for preserving fluorescence staining signal on tissue section and cell smears. It contains anti-fade agent and prevents rapid photobleaching of fluorophores. It is compatible with Medaysis dyes,  DyLight®, FITC, Texas Red, AMCA, Cy®, Alexa Fluor®, fluorescent nuclear stains, fluorescent proteins, fluorescent tracers, and most other fluorophores. The fluorescence is retained during prolonged storage at 4ºC in the dark. It does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, R-PE, PC and APC. 
MedaFluo™ Mounting Medium with DAPI (catalog number MM002M (15ml) and MM002L (100ml)) is fortified with DAPI (4,6-diamidino-2-phenylindole) which counterstains DNA or RNA. This product is to be used in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence.

Features

• Antifade agents prevent photobleaching
  
• Prolonged storage without loss of fluorescent signals
• Optically clear with a refractive index of ~1.40
  

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MedaFluo™ Autofluorescence Blocking Kit

Autofluorescence, caused from endogenous fluorphore such as porphyrins, lipofuscin, NADPH, flavin, collagen, elastin, tryptophan, tyrosine and phenylalanine etc., is an intrinsic property of cells and tissues. It can also be caused by the fixatives used. Autofluorescence complicates the use of fluorescence microscopy and interferes with detection of specific fluorescent signals.
Medaysis ready-to-use  MedaFluo™ Autotofluorescence Blocking Kit (catalog number MB001L (100ml) and MB001M (18ml))will reduce  or eliminate autofluorescence and increase the signal to noise ratio. With the Autofluorescence Blocking Reagent,   researchers   can   clearly    visualize and quantify the target signal without interference from the autofluorescence.

Features

• Clean staining no autofluorescence
  
• Increased signal to noise ratio
• User friendly ready- to-use format
  
• Compatible with commercial fluor
  

DuoFluo™ Non-biotin Immunofluorescence Double Staining Detection Kits
Mouse, Rabbit, Rat Antibodies on Human, Mouse and Rat Tissues or Cell Smears

DuoFluo™ Non-biotin Immunofluorescence Double Staining Detection Kits are designed to simultaneously visualize two primary antibody-antigen complexes on the same tissue section. Autofluorescence Blocking Reagents are included in the kits to reduce/eliminate the autofluorescence on tissue sections to achieve superior signal/noise ratio. Mounting medium which contains DAPI is included and it will produce clear and shiny blue nuclear counterstaining.
The Fluor Dyes exhibit bright fluorescence intensity and photostability. Also it remains highly fluorescent over a broad pH range (pH 4-9). These make it an ideal choice for researcher’s immunofluorescence applications. It uses proprietary fluorescence signal enhancing reagents which also contains agents to reduce non- specific background.