Left: breast carcinoma stained with anti-CK [AE1&AE3] using MedaView™ Two-step Polymer-HRP Detection Kit.

Right: breast carcinoma stained with anti-ER [SP1] using MedaView™ Two-step Polymer-HRP Detection Kit

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Immunohistochemistry (IHC) Detection Systems

MedaView™ One-step and Two-step Polymer-HRP or -AP Anti-Mouse&Rabbit Systems are a biotin-free visualization method used in immunohistochemical protocols. It is an indirect method designed based on proprietary technologies to detect mouse and rabbit primary antibodies on the targeted specimens. The resulting antibody-enzyme can be visualized by HRP- or AP- compatible chromogens and observed by bright field microscopy. This method increases staining sensitivity when compared to other conventional detection methods and eliminates non-specific staining caused by endogenous biotin. These detection systems are compatible with both manual and open automated staining platforms.