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​Medaysis Ultra-Sensitive JAK2 Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the JAK2 gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type.  It is designed to amplify JAK2 gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.


The feature of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required


​Gene Name
JAK2 Janus kinase 2
Catalog
20 tests: JAK20001-20; 50 tests: JAK20001-50
Official Symbol
JAK2
Alternative Name                                                                                             
JAK2; Janus kinase 2; tyrosine-protein kinase JAK2; JTK10; JAK-2; Janus kinase 2 (a protein tyrosine kinase); THCYT3
GeneID
3717
Uniprot ID
O60674
Target Codon
Exon 14: V617
Other Interested Codons
-
Covered Gene Range
Exon 14: 608~621 aa
List of Components1. JAK2 Exon 14 Primer Set
2. JAK2 Exon 14 Sequencing Primer-R
3. DNA Quality Control Primer Mix
4. 2x PCR Master Mix
5. Nuclease-Free Water
Recommended PCR Condition95°C, 10mins;
94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
72°C, 10mins°;
10°C, ∞
Platform
PCR + Sanger sequencing assay
Shipping Condition
4°C
Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles
Datasheet
Ultra-Sensitive JAK2 Mutation Detection Kit


                 
 

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JAK2 Mutation Detection Kit