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KIT Mutation Detection Kit

​Medaysis Ultra-Sensitive KIT Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the KIT gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type.  It is designed to amplify KIT gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.


Features of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required


​Gene Name
KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Catalog
20 tests: KIT0001-0020; 50 tests: KIT0001-50
Official Symbol
KIT
Alternative Name                                                                                             
KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT, piebald trait; mast/stem cell growth factor receptor Kit; C Kit; CD117; SCFR; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; PBT; C-Kit
GeneID
3815
Uniprot ID
P10721
Target Codon

Exon 11: V560/ L576

Exon 13: K642

Other Interested Codons
Exon 11: K550/ K558/ V559/ N564/ G565/ N567/ Y568/ A569/ T574
Exon 13: V643/ Y646/ L647/ M651
Covered Gene Range
Exon 11(A): 551~591 aa
Exon 11(B): 549~584 aa
Exon 13: 635~663 aa
List of Components1. JAK2 Exon 14 Primer Set
2. JAK2 Exon 14 Sequencing Primer-R
3. DNA Quality Control Primer Mix
4. 2x PCR Master Mix
5. Nuclease-Free Water
Recommended PCR Condition95°C, 10mins;
94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
72°C, 10mins°;
10°C, ∞
Platform
PCR + Sanger sequencing assay
Shipping Condition
4°C
Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles
Datasheet
Ultra-Sensitive KIT Mutation Detection Kit

       
 

info@medaysis.com    Toll Free: 877-524-9167