Medaysis Ultra-Sensitive KIT Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the KIT gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type. It is designed to amplify KIT gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.
Features of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required
| Gene Name | KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog |
| Catalog | 20 tests: KIT0001-0020; 50 tests: KIT0001-50 |
| Official Symbol | KIT |
| Alternative Name | KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT, piebald trait; mast/stem cell growth factor receptor Kit; C Kit; CD117; SCFR; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; PBT; C-Kit |
| GeneID | 3815 |
| Uniprot ID | P10721 |
| Target Codon | Exon 11: V560/ L576 Exon 13: K642 |
| Other Interested Codons | Exon 11: K550/ K558/ V559/ N564/ G565/ N567/ Y568/ A569/ T574 Exon 13: V643/ Y646/ L647/ M651 |
| Covered Gene Range | Exon 11(A): 551~591 aa Exon 11(B): 549~584 aa Exon 13: 635~663 aa |
| List of Components | 1. JAK2 Exon 14 Primer Set 2. JAK2 Exon 14 Sequencing Primer-R 3. DNA Quality Control Primer Mix 4. 2x PCR Master Mix 5. Nuclease-Free Water |
| Recommended PCR Condition | 95°C, 10mins; 94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles); 72°C, 10mins°; 10°C, ∞ |
| Platform | PCR + Sanger sequencing assay |
| Shipping Condition | 4°C |
| Long Term Storage | Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles |
| Datasheet | Ultra-Sensitive KIT Mutation Detection Kit |
info@medaysis.com Toll Free: 877-524-9167
Medaysis
Enable Innovation