KIT Mutation Detection Kit

Medaysis Ultra-Sensitive KIT Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the KIT gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type. It is designed to amplify KIT gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.

Features of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required

Gene Name	KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Catalog	20 tests: KIT0001-0020; 50 tests: KIT0001-50
Official Symbol	KIT
Alternative Name	KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT, piebald trait; mast/stem cell growth factor receptor Kit; C Kit; CD117; SCFR; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; PBT; C-Kit
GeneID	3815
Uniprot ID	P10721
Target Codon	Exon 11: V560/ L576 Exon 13: K642
Other Interested Codons	Exon 11: K550/ K558/ V559/ N564/ G565/ N567/ Y568/ A569/ T574 Exon 13: V643/ Y646/ L647/ M651
Covered Gene Range	Exon 11(A): 551~591 aa Exon 11(B): 549~584 aa Exon 13: 635~663 aa
List of Components	1. JAK2 Exon 14 Primer Set 2. JAK2 Exon 14 Sequencing Primer-R 3. 2x PCR Master Mix 4. Nuclease-Free Water
Recommended PCR Condition	95°C, 10mins; 94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles); 72°C, 10mins°; 10°C, ∞
Platform	PCR + Sanger sequencing assay
Shipping Condition	4°C
Long Term Storage	Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles
Datasheet	Ultra-Sensitive KIT Mutation Detection Kit

info@medaysis.com Toll Free: 877-524-9167