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KRAS Mutation Detection Kit

Medaysis

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KRAS ((Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog) gene encodes a small GTPase protein that functions downstream of EGFR-induced cell signaling and participates in the activation of important oncogenic signaling pathways. Oncogenic KRAS gene mutations tend to cause constitutive activation, on which the tumor relies to maintain its growth, apoptosis, angiogenesis and metastasis. Therefore, certain studies have been focused on the relation of KRAS gene mutation on different cancer types and others.


Medaysis Ultra-Sensitive KRAS Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the KRAS gene (V600). Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type.  It is designed to amplify KRAS gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, fine needle biopsies (FNA), pleural effusion specimens, or and plasma samples.


Features of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required 


​Gene Name
KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Catalog
20 tests: KRAS0001-20; 50 tests: KRAS0001-50
Official Symbol
KRAS
Alternative Name                                                                                             
KRAS; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog; KRAS2, v Ki ras2 Kirsten rat sarcoma 2 viral oncogene homolog; GTPase KRas; KRAS1; K-Ras 2; c-Ki-ras; oncogene KRAS2; K-ras p21 protein; c-Kirsten-ras protein; PR310 c-K-ras oncogene.
GeneID
3845
Uniprot ID
P01116
Target Codon

Exon 2: G12 / G13
Exon 3: A59 / Q61

Exon 4: K117 / A146

Covered Gene Range
KRAS Exon 2: 3~23 aa
KRAS Exon 3: 53~96 aa
KRAS Exon 4(A): 107~150 aaKRAS Exon 4(B): 107~150 aa
List of Components1. KRAS Exon 2 Primer Set
2. KRAS Exon 3 Primer Set
3. KRAS Exon 4 Primer Set (A)
4. KRAS Exon 4 Primer Set (B)
5. KRAS Exon 2 Sequencing Primer-R
6. KRAS Exon 3 Sequencing Primer-R
7. KRAS Exon 4 Sequencing Primer-R (A)
8. KRAS Exon 4 Sequencing Primer-R (B)
9. DNA Quality Control Primer Mix
10. 2x PCR Master Mix
11. Nuclease-Free Water
Recommended PCR Condition95°C, 10mins;
94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
72°C, 10mins°;
10°C, ∞
Platform
PCR + Sanger sequencing assay
Shipping Condition
4°C
Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles
Datasheet
Ultra-Sensitive KRAS Mutation Detection Kit