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MPL Mutation Detection Kit

​​​The MPL gene encodes the thrombopoietin receptor protein, which promotes the cell growth and division. Especially, this thrombopoietin receptor is important for the proliferation of megakaryocytes and the maintenance of hematopoietic stem cells within the bone marrow. Mutations of the MPL gene are usually referred to as Exon 10 W515, which have been associated with essential thrombocythemia and primary (idiopathic) myelofibrosis, leading to the overproduction of abnormal megakaryocytes, an increased number of platelets, as well as many signs and symptoms of the condition. (1-3)

Medaysis Ultra-Sensitive MPL Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the MPL gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type (Table 1).  It is designed to amplify MPL gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens. 

The feature of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required

​Gene Name
MPL proto-oncogene, thrombopoietin receptor
20 tests: MPL0101-20
Official Symbol
Alternative Name                                                                                             
Myeloproliferative leukemia protein, Proto-oncogene c-Mpl, CD_antigen: CD110
Uniprot ID
List of Components1. MPL Exon 10 PCR primer mix
2. MPL Exon 10 Seq primer-R
3. 2x PCR Master Mix
4. Nuclease-Free Water
Recommended PCR Condition95°C, 5mins;
95°C, 30seconds–>59°C, 30seconds–>72°C, 1mins (x45 cycles);
72°C, 10mins°;
10°C, ∞
PCR + Sanger sequencing assay
Shipping Condition
Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles
Ultra-Sensitive MPL Mutation Detection Kit    Toll Free: 877-524-9167