NRAS is a member of the RAS oncogene family and plays a role in the pathogensis in various cancers. Activating RAS mutations including KRAS, NRAS and HRAS have been found in 15-25% of human tumors and mainly in condons 12, 13, 59, 61. NRAS mutation accounts for 15% of all RAS mutation and indicates relative resistance to anti-EGFR therapy. Medaysis NRAS Mutation Detection Kit includes the primer set used to amplify NRAS gene and the DNA quality control primers to assure concordance of the test results.​


Medaysis Ultra-Sensitive NRAS Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the NRAS gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type.  It is designed to amplify NRAS gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, fine needle biopsies (FNA), pleural effusion specimens, or and plasma samples.  


Features of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required


​Gene Name
NRAS neuroblastoma RAS viral (v-ras) oncogene homolog
Catalog
20 tests: NRAS0001-20; 50 tests: NRAS0001-50
Official Symbol
NRAS
Alternative Name                                                                                             
NRAS; neuroblastoma RAS viral (v-ras) oncogene homolog; GTPase NRas; N ras; N-ras protein part 4; transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog; NS6; ALPS4; Nras; NRAS1
GeneID
4893
Uniprot ID
P01111
Target Codon

NRAS Exon 2: G12 / G13
NRAS Exon 3: A59 / Q61
NRAS Exon 4: K117 / A146

Covered Gene Range
NRAS Exon 2: 6~37 aa
NRAS Exon 3: 52~97 aa
NRAS Exon 4(A): 108~150 aa
NRAS Exon 4(B): 123~150 aa
List of Components1. NRAS Exon 2 Primer Set
2. NRAS Exon 3 Primer Set
3. NRAS Exon 4 Primer Set (A)
4. NRAS Exon 4 Primer Set (B)
5. NRAS Exon 2 Sequencing Primer-R
6. NRAS Exon 3 Sequencing Primer-R
7. NRAS Exon 4 Sequencing Primer-R (A)
8. NRAS Exon 4 Sequencing Primer-R (B)
9. DNA Quality Control Primer Mix
10. 2x PCR Master Mix
11. Nuclease-Free Water
Recommended PCR Condition95°C, 10mins;
94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
72°C, 10mins°;
10°C, ∞
Platform
PCR + Sanger sequencing assay
Shipping Condition
4°C
Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles
Datasheet
Ultra-Sensitive NRAS Mutation Detection Kit


                 
 

NRAS Mutation Detection Kit

info@medaysis.com    Toll Free: 877-524-9167

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