Ultra-Sensitive Mutation Detection CloDiA™ PCR Kits
Medaysis’ Ultra-Sensitive Mutation Detection Kits provide Clonal Differential Amplifier (CloDiA™) PCR using novel and proprietary primers to amplify somatic mutations and suppress or block the amplification of wild type gene in human genomic DNA.
CloDiA™ PCR includes two types of technique - Unindel™ PCR and Stuntmer™ PCR. Unindel™ PCR is designed to detect a broad range of insertions/deletions (universal insertions/deletions) in the target region. The three-primer set comprising forward primer, reverse primer and blocker inhibits amplification of wild type gene but enables amplification of exonic insertions/deletions.
Stuntmer™ PCR is designed to detect a broad range of point mutations in the target region. Dual port primer of R Port and E Port is designed for self-competition to preferably amply mutated gene but suppress wild type gene amplification.
This sensitive and specific method enables the detection of less than 1% (as little as 20 ng to 100 ng) of) mutant genes mixed with the wild type. Besides known mutations, this method can detect unknown mutations in the target region.
Products by Gene Types SDS
Products by Cancer Types
Breast | EGFR PIK3CA TP53 |
Colorectal | BRAF EGFR HRAS KRAS NRAS PIK3CA TP3 |
Gastric | PIK3CA TP53 |
Leukemia | ABL ASXL1 CALR DNMT3A FLT3 IDH1 JAK2 KIT KRAS NRAS PIK3CA SF3B1 TET2 TP53 |
Liver | BRAF KRAS PIK3CA TP53 |
Lung | BRAF EGFR KIT KRAS PIK3CA TP53 |
Ovary | BRAF EGFR KIT KRAS NRAS PIK3CA TP53 |
Prostate | PIK3CA TP53 |
Medaysis
Enable Innovation
info@medaysis.com Toll Free: 877-524-9167