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Ultra-Sensitive Mutation Detection CloDiA™ PCR Kits

Medaysis’ Ultra-Sensitive Mutation Detection Kits provide Clonal Differential Amplifier (CloDiA™) PCR using novel and proprietary primers to amplify somatic mutations and suppress or block the amplification of wild type gene in human genomic DNA.

CloDiA™ PCR includes two types of technique - Unindel™ PCR and Stuntmer™ PCR. Unindel™ PCR is designed to detect a broad range of insertions/deletions (universal insertions/deletions) in the target region. The three-primer set comprising forward primer, reverse primer and blocker inhibits amplification of wild type gene but enables amplification of exonic insertions/deletions.
Stuntmer™ PCR is designed to detect a broad range of point mutations in the target region. Dual port primer of R Port and E Port is designed for self-competition to preferably amply mutated gene but suppress wild type gene amplification.
 
This sensitive and specific method enables the detection of less than 1% (as little as 20 ng to 100 ng) of) mutant genes mixed with the wild type. Besides known mutations, this method can detect unknown mutations in the target region.

• Detect known and unknown mutations in target Exons
• Sensitive and specific amplification of mutant loci
• Optimized protocol for reliable and reproducible results
• Easy to use, straightforward analysis
  

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