PI3KCA Mutation Detection Kit
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Medaysis Ultra-Sensitive PI3KCA Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the PI3KCA gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type. It is designed to amplify PI3KCA gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.
Features of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required
| Gene Name | PIK3CA phosphoinositide-3-kinase, catalytic, alpha polypeptide |
| Catalog | 20 tests: PIK3CA0001-20; 50 tests: PIK3CA0001-50 |
| Official Symbol | PI3KCA |
| Alternative Name | PIK3CA; phosphoinositide-3-kinase, catalytic, alpha polypeptide; phosphatidylinositol-4,5- bisphosphate 3-kinase catalytic subunit alpha isoform; PI3K; PI3K-alpha; PI3-kinase p110 subunit alpha; ptdIns-3-kinase subunit p110-alpha; serine/threonine protein |
| GeneID | 5290 |
| Uniprot ID | P42336 |
| Target Codon | Exon 9: E542 / E545 Exon 20: H1047 |
| Other Interested Codons | Exon 9: Q546/D549 |
| Covered Gene Range | Exon 9: 536~554 aa Exon 20: 1039~1069 aa |
| List of Components | 1. PIK3CA Exon 9 Primer Set 2. PIK3CA Exon 20 Primer Set 3. PIK3CA Exon 9 Sequencing Primer-R 4. PIK3CA Exon 20 Sequencing Primer-R 5. DNA Quality Control Primer Mix 6. 2x PCR Master Mix 7. Nuclease-Free Water |
| Recommended PCR Condition | 95°C, 10mins; 94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles); 72°C, 10mins°; 10°C, ∞ |
| Platform | PCR + Sanger sequencing assay |
| Shipping Condition | 4°C |
| Long Term Storage | Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles |
| Datasheet | Ultra-Sensitive PI3KCA Mutation Detection Kit |