PI3KCA Mutation Detection Kit

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​Medaysis Ultra-Sensitive PI3KCA Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the PI3KCA gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type.  It is designed to amplify PI3KCA gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.


Features of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required


​Gene Name
PIK3CA phosphoinositide-3-kinase, catalytic, alpha polypeptide
Catalog
20 tests: PIK3CA0001-20; 50 tests: PIK3CA0001-50
Official Symbol
PI3KCA
Alternative Name                                                                                             
PIK3CA; phosphoinositide-3-kinase, catalytic, alpha polypeptide; phosphatidylinositol-4,5- bisphosphate 3-kinase catalytic subunit alpha isoform; PI3K; PI3K-alpha; PI3-kinase p110 subunit alpha; ptdIns-3-kinase subunit p110-alpha; serine/threonine protein
GeneID
5290
Uniprot ID
P42336
Target Codon

Exon 9: E542 / E545

Exon 20: H1047

Other Interested Codons
Exon 9: Q546/D549
Covered Gene Range
Exon 9: 536~554 aa
Exon 20: 1039~1069 aa
List of Components1. PIK3CA Exon 9 Primer Set
2. PIK3CA Exon 20 Primer Set
3. PIK3CA Exon 9 Sequencing Primer-R
4. PIK3CA Exon 20 Sequencing Primer-R
5. DNA Quality Control Primer Mix
6. 2x PCR Master Mix
7. Nuclease-Free Water
Recommended PCR Condition95°C, 10mins;
94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
72°C, 10mins°;
10°C, ∞
Platform
PCR + Sanger sequencing assay
Shipping Condition
4°C
Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles
Datasheet
Ultra-Sensitive PI3KCA Mutation Detection Kit