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SF3B1 Mutation Detection Kit

​Medaysis Ultra-Sensitive SF3B1 Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the SF3B1 gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type.  It is designed to amplify SF3B1 gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.


Features of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required


​Gene Name
SF3B1 splicing factor 3b, subunit 1
Catalog
20 tests: SF3B10001-20; 50 tests: SF3B10001-50
Official Symbol
SF3B1
Alternative Name                                                                                             
SF3B1; splicing factor 3b, subunit 1; Hsh155; Prp10; PRPF10; SAP155; SF3b155; pre-mRNA processing 10; spliceosome associated protein 155; spliceosome-associated protein 1
GeneID
23451
Uniprot ID
A0JLT9
Target Codon

-

Other Interested Codons
-
Covered Gene Range
-
List of Components1. SF3B1 Primer Set
2. SF3B1 Sequencing Primer-R
3. DNA Quality Control Primer Mix
4. 2x PCR Master Mix
5. Nuclease-Free Water
Recommended PCR Condition95°C, 10mins;
94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles);
72°C, 10mins°;
10°C, ∞
Platform
PCR + Sanger sequencing assay
Shipping Condition
4°C
Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles
Datasheet
Ultra-Sensitive SF3B1 Mutation Detection Kit