​Medaysis Ultra-Sensitive TP53 Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the TP53 gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type.  It is designed to amplify TP53 gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.


​Features of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required


​Gene Name
TP53 tumor protein p53
Catalog
20 tests: TP530001-20; 50 tests: TP530001-50
Official Symbol
TP53
Alternative Name                                                                                             
TP53; tumor protein p53; cellular tumor antigen p53; LFS1; Li Fraumeni syndrome; p53; antigen NYCO-13; mutant p53 protein; phosphoprotein p53; p53 tumor suppressor; truncated p53 protein; tumor suppressor TP53; transformation-related protein 53; P53; TRP53
GeneID
7157
Uniprot ID
P04637
Target Codon

Exon 8: R273

Other Interested Codons
Exon 8: C275/ R282
Covered Gene Range
Exon 8: 266~306
List of Components1. TP53 Exon 8 Primer Set
2. TP53 Exon 8 Sequencing Primer-R
3. 2x PCR Master Mix
4. Nuclease-Free Water
Recommended PCR Condition95°C, 5mins;
95°C, 0.5mins–>59°C, 0.5mins–>72°C, 1mins (x45 cycles);
72°C, 10mins°;
10°C, ∞
Platform
PCR + Sanger sequencing assay
Shipping Condition
4°C
Long Term Storage
Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles
Datasheet
Ultra-Sensitive TP53 Mutation Detection Kit

      

info@medaysis.com    Toll Free: 877-524-9167

TP53 Mutation Detection Kit

Medaysis

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