Medaysis Ultra-Sensitive TP53 Mutation Detection Kit is a highly specific and sensitive PCR technique that is able to detect common somatic mutations in the TP53 gene. Used with Sanger sequencing, it can detect less than 1% (as little as 20 ng to 100 ng of) mutant genes mixed with the wild-type. It is designed to amplify TP53 gene from formalin-fixed paraffin-embedded (FFPE) tissues, fresh or frozen tissues, cell smears, fine needle biopsies (FNA) or pleural effusion specimens.
Features of Medaysis mutation detection kit:
1. Detect known and unknown mutation in target Exons
2. Highly specific and sensitive mutation enrich technology
3. Open system and easy to be done
4. Minimal sample size
5. No microdissection required
| Gene Name | TP53 tumor protein p53 |
| Catalog | 20 tests: TP530001-20; 50 tests: TP530001-50 |
| Official Symbol | TP53 |
| Alternative Name | TP53; tumor protein p53; cellular tumor antigen p53; LFS1; Li Fraumeni syndrome; p53; antigen NYCO-13; mutant p53 protein; phosphoprotein p53; p53 tumor suppressor; truncated p53 protein; tumor suppressor TP53; transformation-related protein 53; P53; TRP53 |
| GeneID | 7157 |
| Uniprot ID | P04637 |
| Target Codon | Exon 8: R273 |
| Other Interested Codons | Exon 8: C275/ R282 |
| Covered Gene Range | Exon 8: 266~306 |
| List of Components | 1. TP53 Exon 8 Primer Set 2. TP53 Exon 8 Sequencing Primer-R 3. DNA Quality Control Primer Mix 4. 2x PCR Master Mix 5. Nuclease-Free Water |
| Recommended PCR Condition | 95°C, 10mins; 94°C, 1mins–>65°C, 3mins–>57°C, 1mins–>72°C, 1mins (x45 cycles); 72°C, 10mins°; 10°C, ∞ |
| Platform | PCR + Sanger sequencing assay |
| Shipping Condition | 4°C |
| Long Term Storage | Stable for up to one year when store at -20°C. Avoid freeze/thaw cycles |
| Datasheet | Ultra-Sensitive TP53 Mutation Detection Kit |
info@medaysis.com Toll Free: 877-524-9167
TP53 Mutation Detection Kit
Medaysis
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